Mouse Lutropin subunit beta (LHB) ELISA Kit

Reactivity: Mouse (Mus musculus) UniProt: O09108 Abbreviation: LHB Aliases: CGB4; LSH-B; hLHB; interstitial cell stimulating hormone; beta chain|luteinizing hormone beta subunit|lutropin; beta chain Application: ELISA Range: 8-240 mIU/mL Sensitivity: 3.5 mlU/mL Intra-AssayCV: ?6.2% Inter-AssayCV: ?8.6% Recovery: 1.02 Sample Type: Serum, Plasma, Other biological fluids Detection Method: Sandwich Analysis Method: Quantitative Test principle: This assay employs a two-site sandwich ELISA to quantitate LHB in samples. An antibody specific for LHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LHB bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview: Lutropin subunit beta is a member of the glycoprotein hormone beta chain family and encodes the beta subunit of luteinizing hormone (LH). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and a unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.7. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism. Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).