Mouse Lymphotoxin-beta (LTB) ELISA Kit

Reactivity: Mouse (Mus musculus) UniProt: P41155 Abbreviation: LTB Aliases: DAAP-90L16.4; TNFC; TNFSF3; p33; lymphotoxin-beta|tumor necrosis factor C Application: ELISA Range: 15.6-1000 pg/mL Sensitivity: 6.3 pg/mL Intra-AssayCV: ?3.6% Inter-AssayCV: ?7.2% Recovery: 0.85 Sample Type: Serum, Plasma, Other biological fluids Detection Method: Sandwich Analysis Method: Quantitative Test principle: This assay employs a two-site sandwich ELISA to quantitate LTB in samples. An antibody specific for LTB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLTB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LTB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LTB bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview: Lymphotoxin beta (TNF superfamily, member 3), also known as LTB, is a type II membrane protein of the TNF family. It anchors lymphotoxin-alpha to the cell surface through heterotrimer formation. The predominant form on the lymphocyte surface is the lymphotoxin-alpha 1/beta 2 complex (e.g. 1 molecule alpha/2 molecules beta) and this complex is the primary ligand for the lymphotoxin-beta receptor. The minor complex is lymphotoxin-alpha 2/beta 1. LTB is an inducer of the inflammatory response system and involved in normal development of lymphoid tissue. Lymphotoxin-beta isoform b is unable to complex with lymphotoxin-alpha suggesting a function for lymphotoxin-beta which is independent of lympyhotoxin-alpha. Alternative splicing results in multiple transcript variants encoding different isoforms. Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).