Mouse Matrilin-4 (MATN4) ELISA Kit

Reactivity: Mouse (Mus musculus) UniProt: O89029 Abbreviation: MATN4 Aliases: FLJ14417; HE6WCR54; OTTHUMP00000031671 Application: ELISA Range: Request Information Sensitivity: Request Information Intra-AssayCV: ?4.5% Inter-AssayCV: ?6.3% Recovery: 0.91 Sample Type: Serum, Plasma, Other biological fluids Detection Method: Sandwich Analysis Method: Quantitative Test principle: This assay employs a two-site sandwich ELISA to quantitate MATN4 in samples. An antibody specific for MATN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMATN4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MATN4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MATN4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview: MATN4 encodes a member of von Willebrand factor A domain containing protein family. The proteins of this family are thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. The specific function of this gene product has not yet been determined. Three alternatively spliced variants have been described. The human matrilin-4 gene contains 10 exons and spans approximately 12 kb. Alternative splicing leads to 3 different transcripts encoding protein isoforms that all contain a putative signal peptide, 2 vWFA-like domains, and the coiled-coil region. The isoforms differ in that they include either 1, 2, or 3 EGF-like domains. There are 4 EGF-like domains in mouse matrilin-4, but the first EGF-like domain in the human protein is not expressed. Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).